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Your present location:Home/Products/DNA&RNA Purification/RNA/Microbial RNA/HiPure Yeast & MicroBiol RNA Kit
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HiPure Yeast & MicroBiol RNA Kit

R4182
CAT NO PRODUCT NAME SIZE PRICE
R418202 HiPure Yeast & MicroBiol RNA Kit 50 preps $249.00
R418203 HiPure Yeast & MicroBiol RNA Kit 250 preps $1,144.00

Introduction

This kit uses phenol and bead grinding method, which is suitable for extracting high purity total RNA from yeast, bacteria, fungal mycelium or sporangium powder, and other difficult lysed microorganisms. The kit is based on silica gel column purification technology, the whole extraction process only takes about 40 minutes. The purified RNA can be directly used for RT-PCR, Northern blot, Poly-A + purification, nucleic acid protection and in vitro translation experiments, ect.

Details

Specifications

Features Specifications
Main Functions Isolation total RNA from bacteria, yeast cells
Applications RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Bacteria, yeast cells
Sample amount Bacteria: <10^9;Yeast cells:<1 x10^7
Elution volume ≥30μl
Time per run ≤40 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg

Kit Contents

Contents R418202 R418203
Purification Times 50 Preps 250 Preps
gDNA Filter Mini Column 50 250
HiPure RNA Mini Column I 50 250
2ml Collection Tubes
100
500
2ml Bead Tubes F
50
250
Buffer ATL 30 ml 150 ml
Buffer PCI 30 ml 150 ml
Buffer GDP 20 ml 100 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml

Storage and Stability

Buffer PCI should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

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