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Unlocking the Potential of Circulating Free DNA with Magen Biotech's HiPure Circulating DNA Midi Kit

Release date:2024-05-29

At Magen Biotech, we're committed to advancing biological research and diagnostics with innovative solutions. Our HiPure Circulating DNA Midi Kit C, incorporating Vacuum Protocol, is a testament to our dedication to enabling efficient purification of circulating nucleic acids from human plasma, serum, or urine.

Understanding Circulating Free DNA

circulating free DNA (cfDNA) represents a remarkable avenue for research and diagnostic applications. These nucleic acids, including tumor-specific extracellular DNA fragments and fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). Harnessing the potential of cfDNA opens doors to a myriad of possibilities in fields ranging from cancer research to non-invasive prenatal testing.

The HiPure Advantage

Our HiPure Circulating DNA Midi Kit employs state-of-the-art silica column purification technology. This innovative approach ensures:

- High Yield: Optimal processes guarantee maximum recovery of free DNA, facilitating downstream applications.
- High Concentration: Low elution volumes maintain high nucleic acid concentrations, enhancing assay sensitivity.
- High Purity: Advanced methods effectively remove inhibitors and protein contaminants, ensuring pristine nucleic acid samples.
- High Recovery: Silica gel column purification achieves remarkable DNA recovery rates, empowering robust research outcomes.

Streamlined Workflow for Enhanced Efficiency

The kit's purification process is straightforward and efficient:

1. Sample Lysis and Digestion: The sample is lysed and digested, releasing nucleic acids into the lysate.

2. Adsorption and Filtration: The lysate is transferred to an adsorption column, where nucleic acids are selectively adsorbed on the membrane, while proteins are removed through filtration.

3. Washing and Elution: After thorough washing to remove impurities, nucleic acids are eluted with a low-salt buffer, ready for downstream applications.

Comprehensive Kit Contents for Seamless Operations

Our kit comes equipped with all necessary components for successful purification:

- Purification buffers and reagents.
- Mini columns for silica-based purification.
- Collection tubes and extenders for sample handling.
- Vac-connectors for vacuum-based protocols.

Storage and Stability

For convenience and flexibility, our kit components are designed for stable storage and usage:

- Proteinase K and Carrier RNA can be stored at 2-8°C, with short-term room temperature storage (15-25°C) not affecting performance.
- Remaining kit components can be stored dry at room temperature (15-25°C) for at least 18 months, ensuring long-term stability and usability.

Conclusion

With Magen Biotech's HiPure Circulating DNA Midi Kit C, researchers and clinicians gain access to a reliable and efficient solution for purifying circulating free DNA. Whether exploring cancer biomarkers or conducting non-invasive prenatal testing, our kit empowers users with the tools they need to unlock the potential of cfDNA. Trust Magen Biotech for cutting-edge solutions in nucleic acid purification and biological sample preparation.

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