As a leading name in biological sample preparation, Magen Biotech is proud to introduce our innovative HiPure Plasmid Mini Kit, offering a streamlined solution for plasmid DNA minipreps. Plasmid preparation is a critical step in molecular biology research, and our kit simplifies this process while ensuring high yields of pure DNA.
Introduction to HiPure Plasmid Mini Kit
Our HiPure Plasmid Mini Kit utilizes advanced silica membrane technology to streamline plasmid DNA purification. Say goodbye to the cumbersome steps associated with loose resins or slurries. With our kit, plasmid DNA purification becomes fast, simple, and cost-effective. Phenol extraction and ethanol precipitation are no longer necessary, and you can obtain high-quality plasmid DNA ready for immediate use.
Specifications and Features
- Main Functions: Our kit isolates up to 35μg of plasmid DNA from 1-5ml bacterial culture, making it suitable for various applications such as enzyme digestion, sequencing, PCR, and cloning.- Purification Method: Mini spin column
- Purification Technology: Silica technology
- Process Method: Manual (centrifugation or vacuum)
- Sample Type: Conventional plasmid, plasmid less than 30KB
- Sample Amount: High copy plasmid: 1-5ml culture medium, Low copy number plasmid: 5-10ml culture medium
Principle Behind HiPure Plasmid Mini Kit
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. Our kit's unique silica membrane completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure involves three basic steps: preparation and clearing of a bacterial lysate by alkaline method, transfer of the supernatant to the column to bind DNA, and elution of nucleic acid with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA) after washing proteins and other impurities.
Applications
The HiPure Plasmid Mini Kit from Magen Biotech is revolutionizing plasmid preparation in molecular biology labs worldwide. With its simplicity, speed, and efficiency, researchers can optimize their workflows and focus more on their scientific discoveries. Whether you're conducting enzyme digestion, sequencing, PCR, or cloning, our kit provides reliable results every time.
Conclusion
Experience the difference with Magen Biotech's HiPure Plasmid Mini Kit. Simplify your plasmid preparation process today and elevate your research to new heights. For more information and to purchase our kit, visit our website or contact us directly. With Magen Biotech, achieving high-quality plasmid DNA has never been easier. Unlock the potential of your research with our innovative solutions.