At Magen Biotech, we take pride in providing the industry with high-quality products for nucleic acid extraction. Our core raw material, the 6M Guanidine Hydrochloride, plays a pivotal role in enabling efficient extraction processes. With stringent screening and quality control measures in place, we ensure that our customers receive a reliable solution for their nucleic acid extraction needs.
Solubility Promotion and Protein Denaturation
Facilitating Solubility of Hydrophobic Molecules
The powerful denaturing properties of our 6M Guanidine Hydrochloride enhance the solubility of hydrophobic molecules, enabling effective extraction. By disrupting intermolecular non-covalent interactions, such as hydrogen bonds and hydrophobic bonds, it promotes the dissolution of otherwise insoluble compounds.
Denaturing Proteins for Successful Extraction
Our Guanidine Hydrochloride effectively denatures proteins, breaking down their secondary and tertiary structures. This denaturation process ensures the removal of proteins and facilitates the extraction of target nucleic acids.
Impact on Protein Structure and Enzymatic Activity
The ordered "secondary structure" of proteins is destroyed, and the peptide chain is stretched in a 6M Guanidine Hydrochloride solution.
This structural alteration affects the enzymatic activity of proteins, rendering them inactive and enabling their removal during the extraction process.
Applications of 6M Guanidine Hydrochloride
Protein Removal: Cleaning Solution and Binding Solution
Our 6M Guanidine Hydrochloride serves as an effective cleaning solution (>3M) and binding solution (4-6M) for protein removal. It ensures the purity of extracted nucleic acids by eliminating unwanted protein contaminants.
Plasmid Extraction: Leveraging the Power of Guanidine Hydrochloride
The denaturing properties of our Guanidine Hydrochloride are particularly valuable in plasmid extraction procedures.
It aids in breaking down cell membranes and releasing plasmid DNA, facilitating downstream applications.
Genomic DNA and Total RNA Extraction: Lysis Solution for Efficient Extraction
Our Guanidine Hydrochloride serves as a lysis solution, enabling efficient extraction of genomic DNA and total RNA. It disrupts cellular structures and releases nucleic acids, ensuring high yields and quality.
Advantages of Magen Biotech's Guanidine Hydrochloride
Low Absorbance in A260, A280, and A230 Measurements
Our Guanidine Hydrochloride exhibits low absorbance in A260, A280, and A230 measurements, minimizing interference during nucleic acid quantification. This allows for accurate and reliable measurements, ensuring precise analysis.
Achieving an Ideal A260/A230 Ratio in Nucleic Acid Products
Molecular Biology Level: White Crystal Appearance:
Magen Biotech's Guanidine Hydrochloride is available in the form of white crystals. This distinctive appearance signifies its molecular biology level and assures users of its quality and consistency.
Conclusion
Magen Biotech's 6M Guanidine Hydrochloride is a versatile and indispensable component in nucleic acid extraction. With its solubility-promoting and protein-denaturing properties, it enables successful extraction processes across various applications. Our Guanidine Hydrochloride offers distinct advantages, including low absorbance, ideal A260/A230 ratio, and a recognizable white crystal appearance. Trust Magen Biotech to unlock the full potential of nucleic acid extraction with our high-quality Guanidine Hydrochloride.