At Magen Biotech, we understand the importance of efficient and reliable purification of free circulating DNA for clinical in vitro detection. Free circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs, play a crucial role in various medical applications. We are proud to introduce our HiPure Circulating DNA Midi Kit, specifically designed to enable the efficient purification of circulating nucleic acids from human plasma, serum, or urine.
Introduction to Free Circulating DNA
Free circulating DNA refers to the presence of DNA fragments in bodily fluids, such as blood or urine, that are not associated with cells. These fragments can provide valuable information about various medical conditions, including cancer and pregnancy. For example, tumor-specific extracellular DNA fragments can help in the early detection and monitoring of cancer, while fetal nucleic acids in maternal blood can be used for noninvasive prenatal testing (NIPT).
Advantages of HiPure Circulating DNA Midi Kit
Our HiPure Circulating DNA Midi Kit offers several key advantages that make it the ideal choice for efficient purification of free circulating DNA:
High Yield: Our kit utilizes the most optimal process, ensuring that free DNA fragments (>50bp) can be obtained to the maximum extent. This high yield of purified DNA allows for more accurate and reliable detection of target sequences.
High Concentration: With our kit, low elution volume is required, resulting in high nucleic acid concentration. This high concentration is crucial for downstream applications that require a sufficient amount of purified DNA.
High Purity: The kit utilizes a low alcohol binding method, effectively removing inhibitors and protein contaminants. This ensures that the purified DNA is of high purity, free from any impurities that may interfere with downstream analysis.
High Recovery: Our kit utilizes silica gel column purification, allowing for the recovery of DNA at the level of picograms. This high recovery rate ensures that even small amounts of free circulating DNA can be successfully purified and utilized for further analysis.
Storage and Stability of the Kit
To ensure the optimal performance of our HiPure Circulating DNA Midi Kit, it is important to follow proper storage and stability guidelines. Proteinase K and Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect their performance. The remaining kit components can be stored dry at room temperature and are stable for at least 18 months. The entire kit can be stored at 2-8°C, but the buffers should be redissolved before use. It is important to ensure that all buffers are at room temperature when used to maintain the integrity and efficacy of the kit.
Conclusion
Magen Biotech's HiPure Circulating DNA Midi Kit offers an efficient and reliable solution for the purification of free circulating DNA. With its high yield, concentration, purity, and recovery rates, our kit enables accurate and sensitive detection of target sequences in clinical samples. We are committed to providing high-quality products that meet the needs of the scientific and medical communities. Trust Magen Biotech for all your free circulating DNA purification needs.