| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D331201 | SolPure Tissue DNA Kit | 1 g | $75.00 |
| D331202 | SolPure Tissue DNA Kit | 5 g | $249.00 |
|
D331203 |
SolPure Tissue DNA Kit |
50 g |
$1,175.00 |
This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue using economic salt out method |
| Applications | PCR, enzyme digestion, Southern hybridization,etc. |
| Purification technology | Salting out method |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissue |
| Sample amount | Unlimited |
| Elution volume | ≥100μl |
| Time per run | Variation with sample amount |
Principles
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
| Contents | D331201 | D331202 |
D331203 |
| Purification Times | 1 g | 5 g |
50 g |
| Proteinase K | 330 µl | 1.8 ml |
18 ml |
| Buffer WTL | 33 ml | 160 ml |
2 x 800 ml |
| Buffer PPS | 12 ml | 55 ml |
500 ml |
|
RNase Solution |
330 µl | 1.8 ml |
18 ml |
| Buffer TE | 12 ml | 60 ml |
200 ml |
RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
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