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HiPure Fastfilter Plasmid Maxi Kit

P1014
CAT NO PRODUCT NAME SIZE PRICE
P101402 HiPure Fastfilter Plasmid Maxi Kit 10 preps $199.00
P101403 HiPure Fastfilter Plasmid Maxi Kit 50 preps $746.00

Introduction

The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.

Details


Specifications

Features Specifications
Main Functions Isolation up to 1mg plasmid DNA from 200ml bacterial culture
Applications Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification method Maxi spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Conventional plasmid, plasmid less than 30KB
Sample amount
100-200ml
Yield 0.4-1mg
Elution volume 500μl
Time per run 60 minutes
Liquid carrying volume per column 20ml
Binding yield of column 1mg


Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).


Advantages

  • High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
  • Fast - it takes only 15-60 minutes to complete the isolation
  • Economy - high cost performance


Kit Contents

Contents P101402 P101403
Purification Times 10 Preps 50 Preps
RNase A 20 mg 40 mg
Buffer P1 140 ml 2 x 350 ml
Buffer P2
140 ml
2 x 350 ml
Buffer LEN3
70 ml
350 ml
Buffer GBT
120 ml
550 ml
Buffer PW1
60 ml
300 ml
Buffer PW2* 50 ml 4 x 100 ml
Elution Buffer 20 ml 120 ml
HiPure DNA Maxi Columns III 10 50
Lysate Clear Maxi Syringe 10 50
50 ml Collection Tubes
20
100


Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37 to dissolve. After addition of RNase ABuffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name CAT NO Features

Sample

amount

Elution

volume

Yield

HiPure Plasmid Mini Kit P1001 Classic, Regular application 1-5ml LB 30-50μl

5–35µg/5ml

HiPure Plasmid Mini Kit II P1002 Classic, Regular application
5-15ml LB 75-100μl

20-80µg/15ml

HiPure Plasmid Plus 96 Kit P1006 Classic, Regular application
1-1.5ml LB 70-150μl

1-15μg/1ml

HiPure Fastfilter Plasmid Midi Kit P1013 Fast
30-50ml LB 0.3-0.8ml

50–250µg/50ml

HiPure Fastfilter Plasmid Maxi Kit
P1014
Fast
100-200ml LB
0.5-1.5ml

0.4–1mg/200ml

Low endotoxin/Endotoxin-free Plasmid
HiPure Plasmid EF Maxi Kit P1156 Classic method, General for all kinds of vectors

100-200ml LB

≥0.7ml 200–1500µg
HiPure Plasmid EF Mini Kit P1154 Recommend for low copy vector, Thoroughly remove RNA

5-15ml LB

≥60μl 10–80µg
HiPure Plasmid EF 96 Kit P1157 Recommend for low copy vector, Thoroughly remove RNA

1-5ml LBx96

≥75μl 30µg
HiPure Plasmid EF Midi Kit P1231 Recommend for High copy vector, Low elution volume, High concentration

25-50ml LB

≥100μl 10-500μg
HiPure Plasmid EF Mega Kit P1116 Recommend for High copy vector(>3μg/ml)

500ml LB

≥2ml 1-10mg

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit -- P1156 ←click here

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