Introduction
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
MagPure Viral DNA/RNA Precast Kit
IVD5412-F-96
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue, cell |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
|
Adaptive instrument |
Nucleic acid extractor, pipetting workstation |
| Sample type | Tissues, cells, lymphocytes and other clinical sample |
| Sample amount |
Cells grown in suspension:3~5 x 106 Animal tissue: 10~20mg Plant tissue: ≤100mg |
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water
Advantages
Kit Contents
| Contents | IVD3020 |
| Purification Times | 200 Preps |
|
MagPure RNA Particles |
7 ml |
|
DNase I |
4 x 600 µl |
|
DNase Buffer |
80 ml |
|
RTL Lysis Buffer |
150 ml |
|
Buffer MCB* |
75 ml |
| Buffer MW1* | 110 ml |
|
Buffer MW2* |
50 ml |
|
RNase Free Water |
60 ml |
| Cat.No |
Reagent |
IVD3020-F-96 |
| DNase Buffer |
|
60 ml |
|
DNase I |
|
2 x 600 μl |
| RTL Lysis Buffer |
|
80 ml |
| Buffer MCB |
|
18 ml |
| 96-Tip |
|
1 |
| Sample plate (DW Plate) |
500μl Buffer MCB |
1 |
| Wash 1 Plate (DW Plate) |
700μl Buffer MW1 30μl MagPure RNA Partilces |
1 |
|
DNase Plate |
Empty |
|
| Wash 2 Plate (DW Plate) |
700μl Buffer MW1 |
1 |
| Wash 3 Plate (DW Plate) |
900μl Buffer MW2 |
1 |
| Elution plate (DW Plate) |
80μl RNase Free Water |
1 |
| Cat.No |
Reagent |
IVD3020-TL-06 |
| Purification times |
|
96 Preps |
| DNase I |
|
2 x 600 μl |
| DNase Buffer |
|
60 ml |
| RTL Lysis Buffer |
|
60 ml |
|
Buffer MCB |
|
40 ml |
| 96-Tip |
|
12 PCS |
|
2.0ml V-bottom plate
|
Row 1/7：500μl Buffer MCB Row 2/8：500μl Buffer MW1 Row 3/9：empty Row 4/10：30μl Magpure RNA Particles
500μl Buffer MW2
Row 5/11：900μl Buffer MW2
Row 6/12：80μl RNase Free Water |
6 |
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
D3018
IVD5412
IVD4173
IVD3020
R4011
D6364
D3141
D3146
D6351
D3187
D3125
D3126
IVD5432
D3182C
IVD3102
D3111