| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D211102 | HiPure Gel DNA Mini Kit | 100 preps | $60.00 |
| D211103 | HiPure Gel DNA Mini Kit | 250 preps | $150.00 |
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
| Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Agarose gel, PCR products, enzyme products |
|
Sample amount |
Agarose gel: ≤500mg |
| Recovery | ≥80% |
| Elution volume | ≥15μl |
| Time per run | ≤20 minutes（1-24 samples） |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 35µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D211102 | D211103 |
| Purification Times | 100 Preps |
250 Preps |
| Buffer GDP | 120 ml |
250 ml |
| Buffer DW2 | 50 ml |
2 x 50 ml |
|
Elution Buffer |
20 ml |
30 ml |
|
HiPure DNA Mini Columns II |
100 |
250 |
| 2 ml Collection Tubes | 100 |
250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Experiment Data
D3018
IVD5412
IVD4173
IVD3020
R4011
D6364
D3141
D3146
D6351
D3187
D3125
D3126
IVD5432
D3182C
IVD3102
D3111
