CN
111.png
banner
Your present location:Home/Products/DNA&RNA Purification/RNA/Plant RNA/HiPure Plant RNA Midi Kit
  • Plant Root RNA extraction.png

HiPure Plant RNA Midi Kit

R4152
CAT NO PRODUCT NAME SIZE PRICE
R415202 HiPure Plant RNA Midi Kit 20 preps $398.00
R415203 HiPure Plant RNA Midi Kit 100 preps $1,740.00

Introduction

The HiPure Plant RNA Midi Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 1mg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from <1g simple plant sample without chloroform
Applications RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method Midi spin column
Purification technology Silica technology, DNA filtration technology
Process method Manual (centrifugation or vacuum)
Sample type Plant leaves, roots, stems, fruits, fungi, etc.
Sample amount
0.5-1 g
Elution volume 400μl
Time per run ≤40 minutes
Liquid carrying volume per column 4ml
Binding yield of column 1mg


Principle

The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.


Advantages

  • Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - medium isolation of several samples can be completed in 40 minutes
  • Safe - no phenol chloroform extraction required
  • Sensitive - RNA can be recovered at the level of PG
  • Broad spectrum - various types of plant samples can be processed by diversity of operating procedures


Kit Contents

Contents R415202 D415203
Purification Times 20 Preps 100 Preps
HiPure DNA Midi Column II
20
100
HiPure RNA Midi Columns II 20 100
15ml Collection Tubes 40 200
Buffer RLC
120 ml
550 ml
Buffer PRC1
120 ml
550 ml
Buffer RW1
70 ml
400 ml
Buffer RW2*
20 ml
100 ml
RNase Free Water
30 ml
120 ml


Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.

Purchase Guide

1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.

2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.

3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.

4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.


Select the right purification kit to get impactful results

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

Articles

Leave A Message

Leave A Message

    Please provide us with the information below, and we'll contact you as soon as possible.

This site uses cookies to improve your online experience,allow you to share content on social media,measure traffic to this website and display customised ads based on your browsing activity.