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DNase I

C12131
CAT NO PRODUCT NAME SIZE PRICE
C12131 DNase I 100 mg $182.00
C12132 DNase I 1 g $1,300.00

Introduction

Bovine pancreatic deoxyribonuclease (also known as DNase) is an endonuclease. It acts on the phospholipid bond, especially the bond adjacent to pyrimidine nucleoside, to produce polynucleotides with free hydroxyl at the 3 'end and phosphate at the 5' end. The optimum pH value of is 7.8. DNase can be activated by divalent metals and inhibited by chelates such as EDTA and sodium dodecyl sulfate. 5 mM calcium ion can be used as a stabilizer to protect DNase from being decomposed by hydrolase. This product is extracted from bovine pancreas and prepared by chromatography to remove the pollution of other hydrolases.

Details


Specifications

Features Specifications
Molecular weight 32KDa
Isoelectric point 8.9
pH range 4.0 - 10.0
Specific activity 3000 kunitz units/mg
Preservation conditions It is recommended to store at -20°C to ensure the stability of activity to the greatest extent (normal temperature transportation or storage will not reduce enzyme activity).
Solution
50mM Tris (pH7.4),10mM CaCl250% (v/v) glycerol
Recommended application
Remove DNA
RNase Residue
Not detection
RNase-Free DNase I Prepare
Dissolve DNase I at 1 mg/ml in 0.1M iodoacetic acid plus 0.15M sodium acetate at a final pH of 5.3. The solution is then heated 40-60 minutes at 55°C and cooled. Finally, 1M CaCl2 is added to the solution to 5mM. Store frozen in small aliquots.


Advantages

  • Pure natural protein, from bovine pancreas
  • High activity and purity, >3000 kunitz units/mg
  • High cost performance


Ordering information

Contents C12131
C12132
Dnase I, Lyophilizate, >3000 units/mg protein 100 mg/Bottle
1 g/Bottle

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