Introduction
Bovine pancreatic deoxyribonuclease (also known as DNase) is an endonuclease. It acts on the phospholipid bond, especially the bond adjacent to pyrimidine nucleoside, to produce polynucleotides with free hydroxyl at the 3 'end and phosphate at the 5' end. The optimum pH value of is 7.8. DNase can be activated by divalent metals and inhibited by chelates such as EDTA and sodium dodecyl sulfate. 5 mM calcium ion can be used as a stabilizer to protect DNase from being decomposed by hydrolase. This product is extracted from bovine pancreas and prepared by chromatography to remove the pollution of other hydrolases.