Introduction
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen's columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
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Features
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Specifications
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Recommended application
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gDNA and RNA Isolation
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Preservation conditions
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Room temperature
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Stability
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Up to 4 years
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Filter membrane
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High quality glass fiber filter GF/B, 2 layers
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Membrane aperture
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1.0μm
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Maximum binding yield of plasmid
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30 μg
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Maximum yield of alcohol mediated Binding
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100 μg
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Single liquid carrying capacity of column
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900 μl
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Minimum elution volume
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80 μl
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Withstand centrifugal force
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4,000-5,000 x g
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Centrifuge
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Low speed centrifuge, Swing out Rotor, can placed a height of 6.5cm square, (height of HiPure DNA Plate & 1.6ml Collection Plate: height, 6.2cm)
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Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
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CAT.No.
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Product Name
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Package
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C13131
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HiPure gDNA Plate (2 x GF/B)
with 1.6ml Collection Plate
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10/Bag
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