Introduction
This product is suitable for rapid extraction of RNA from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
MagPure Blood RNA Kit
R6611
| CAT NO | PRODUCT NAME | SIZE | PRICE |
| IVD3020-F-96 | MagPure Universal RNA Precast Kit (96 channel machine) | 96 preps | $550.00 |
This product is suitable for rapid extraction of RNA from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue, cell |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
|
Adaptive instrument |
Nucleic acid extractor, pipetting workstation |
| Sample type | Tissues, cells, lymphocytes and other clinical sample |
| Sample amount |
Cells grown insuspension:≤1 x 107 Animal tissue: ≤20mg
Plant tissue: ≤100mg
Whole blood: ≤1.5ml |
| Yield |
2-50μg |
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Advantages
Kit Contents
| Cat.No |
Reagent |
IVD3020-F-96 |
| DNase Buffer |
|
60 ml |
|
DNase I |
|
2 x 600 μl |
| RTL Lysis Buffer |
|
80 ml |
| Buffer MCB |
|
18 ml |
| 96-Tip |
|
1 |
| Sample plate (DW Plate) |
500μl Buffer MCB |
1 |
| Wash 1 Plate (DW Plate) |
700μl Buffer MW1 30μl MagPure RNA Partilces |
1 |
|
DNase Plate |
Empty |
|
| Wash 2 Plate (DW Plate) |
700μl Buffer MW1 |
1 |
| Wash 3 Plate (DW Plate) |
900μl Buffer MW2 |
1 |
| Elution plate (DW Plate) |
80μl RNase Free Water |
1 |
Storage and Stability
DNase I should be shipped with ice pack and stored at -20°C after arrival. However, short-term storage (up to 1 week) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15–25°C) for 18 months.
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