CN
111.png
banner
Your present location:Home/Products/DNA&RNA Purification/DNA/Microbial DNA/Column Kits/HiPure Yeast DNA Kit
  • Yeast dna isolation.jpg

HiPure Yeast DNA Kit

D3147
CAT NO PRODUCT NAME SIZE PRICE
D314702 HiPure Yeast DNA Kit 50 preps $154.00
D314703 HiPure Yeast DNA Kit 250 preps $694.00

Introduction

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 107) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from yeast cultures
Applications PCR, southern blot and enzyme digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Yeast culture
Sample amount

Bacterial culture: 1-1.5 ml

Elution volume ≥30μl
Time per run 60 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).

Advantages

  • Fast - several samples can be extracted in 40 minutes (after digestion)
  • High purity - purified DNA can be directly used in various downstream applications
  • Good repeatability - silica technology can obtain ideal results every time
  • High recovery - DNA can be recovered at the level of PG
  • Sufficient components - lysozyme, protease K, RNase A and glass beads

Kit Contents

Contents D314702 D314703
Purification Times 50 Preps 250 Preps
Hipure DNA Mini Columns I 50 250
2ml Collection Tubes 50 250
Glass Beads (0.4~0.6mm)
20 g
90 g
Buffer SE
30 ml
150 ml
Lyticase
1.8 ml 5 x 1.8 ml
Buffer ATL
30 ml
150 ml
ReagentDX
500 μl
1500 μl
Buffer DL 30 ml 150 ml
Buffer GW1*
13 ml
66 ml
Buffer GW2*
20 ml
2 x 50 ml
Proteinase K 12 mg 60 mg
Protease Dissolve Buffer 1.8 ml 5 ml
Buffer AE
15 ml
30 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.

Related Products

Leave A Message

Leave A Message

    Please provide us with the information below, and we'll contact you as soon as possible.

This site uses cookies to improve your online experience,allow you to share content on social media,measure traffic to this website and display customised ads based on your browsing activity.