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Your present location:Home/Products/DNA&RNA Purification/RNA/Microbial RNA/MagPure Stool RNA Kit
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MagPure Stool RNA Kit

R6626
CAT NO PRODUCT NAME SIZE PRICE
R662601 MagPure Stool RNA Kit 48 preps $348.00
R662602 MagPure Stool RNA Kit 96 preps $597.00
R662603 MagPure Stool RNA Kit
5 x 96 preps
$2686.00

Introduction

This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.

Details

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.


Kit Contents

Contents R662601 R662602 R662603
Purification Times 48 Preps 96 Preps
5 x 96 Preps
MagPure RNA Particles 1.7 ml 4.0 ml 18 ml
DNase I 600 μl
2 x 600 μl
10 x 600 μl
DNase Buffer 30 ml
40 ml
200 ml
Buffer SPL 30 ml 60 ml
270 ml
Buffer PHC
30 ml 60 ml
270 ml
Buffer MCB*
9 ml
15 ml
75 ml
Buffer ALB3*
10 ml
20 ml
100 ml
Buffer GW1*
44 ml
66 ml
2 x 220 ml
Buffer RW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
30 ml
120 ml
2ml Bead Tubes
48
96
5 x 96


Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

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