| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D316402 | HiPure SF Plant DNA Kit | 50 preps | $99.00 |
| D316403 | HiPure SF Plant DNA Kit | 250 preps | $447.00 |
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from100 mg simple plant |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount |
Fresh / frozen plant samples: ≤100mg gDried plant / seed samples: ≤20mg |
| Elution volume | ≥30μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
| Contents | D316402 | D316403 |
| Purification Times | 50 Preps | 250 Preps |
|
RNase A |
10 mg |
50 mg |
|
Protease Dissolve Buffer |
1.8 ml |
5 ml |
| Buffer SPL | 30 ml | 150 ml |
| Buffer PS | 10 ml | 50 ml |
|
Buffer GW1 |
22 ml |
110 ml |
| Buffer GW2* | 12 ml | 2 x 50 ml |
|
Buffer AE |
15 ml |
60 ml |
|
HiPure gDNA Mini Columns |
50 |
2 x 125 |
|
2 ml Collection Tubes |
50 |
2 x 125 |
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
| Name | CAT NO |
Fresh / frozen tissue amount |
Dried tissue amount |
Column type |
Elution volume |
Yield (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 - 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 - 3ml | 75-1250μg |
|
HiPure SF Plant DNA Kit |
D3164 |
100mg |
20mg |
1.5ml column |
50 - 100μl |
3-50μg |
|
HiPure SF Plant DNA 96 Kit |
D3167 |
50mg |
15mg |
96 well plate |
75 - 150μl |
3-30μg |
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
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