This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from blood, buffy coat, tissue and other samples |
| Applications | Second generation sequencing, PCR, real time PCR, etc. |
|
Purification method |
Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Sample amount | Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg |
| Yield | 0.1 - 50μg |
| Elution volume |
|
| Time per run |
|
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
| Contents | IVD3102 |
| Purification Times | 200 |
| MagPure Particles | 5 ml |
| Proteinase K | 100 mg |
| Protease Dissolve Buffer | 10 ml |
| Rnase A | 40 mg |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
|
Buffer BD* |
20 ml |
|
Buffer BW1* |
110 ml |
| Elution Buffer | 30 ml |
| Cat.No |
Reagent |
IVD3102-F-96 |
| Proteinase K |
|
50 mg |
|
Protease Dissolve Buffer |
|
6 ml |
| RNase A |
|
20 mg |
| Buffer ATL |
|
30 ml |
| Buffer AL |
|
30 ml |
| 96-Tip |
|
1 |
| Sample plate (DW Plate) |
450µl Buffer BD(Ethanol Added) |
1 |
| Wash 1 Plate (DW Plate) |
600µl Buffer BW1(Ethanol Added) |
1 |
| Wash 2 Plate (DW Plate) |
600µl Buffer BW1(Ethanol Added) |
1 |
| Wash 3 Plate (DW Plate) |
750µl Buffer GW2, 20µl MagPure Particle |
1 |
| Elution plate (DW Plate) |
80µl Elution Buffer |
1 |
| Cat.No |
Reagent |
IVD3102-TL-06 |
| Proteinase K |
|
50 mg |
| RNase A |
|
20 mg |
| Protease Dissolve Buffer |
|
6 ml |
| Buffer ATL |
|
40 ml |
|
Buffer AL |
|
40 ml |
| AS-Tip |
|
12 |
|
2.0ml V-bottom plate
|
Row 1/7：450µl Buffer BD Row 2/8：450µl Buffer BW1 Row 3/9：450µl Buffer BW1 Row 4/10：20μl Magpure Particle
450μl Wash Buffer GW2
Row 5/11：450μl Wash Buffer GW2
Row 6/12：80µl Elution Buffer |
6 |
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
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