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MagPure Pathogen DNA/RNA Enrich Kit

R6672C
CAT NO PRODUCT NAME SIZE PRICE
R667202C MagPure Pathogen DNA/RNA Enrich Kit 96 preps $760.00

Introduction

This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, mNGS/tNGS and other related experiments.

Details


Specifications

Features Specifications
Main Functions Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS/tNGS application, remove host background nucleic acid.
Applications Real Time PCR, biochip analysis, NGS
Products Pathogen DNA / RNA
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution
Sample amount 0.5 - 1.5 ml
Adaptive instrument
Nucleic acid extractor, pipetting workstation


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.


Kit Contents

Contents R667200C R667202C
Purification Times 24 Preps 96 Preps
2ml Bead Tube (0.4g) 24 96
DNase I (Powder) 10 mg
15 mg
DNase Buffer 5 ml
20 ml
Protease Dissolve Buffer 3 ml 8 ml
Lysis Buffer LBX1
40 ml
180 ml
Buffer TL
5 ml
20 ml
Proteinase K
24 mg
120 mg
MagBind Particles N9
1.2 ml
5 ml
Buffer MLB
30 ml
120 ml
Buffer MW1*
13 ml
110 ml
Buffer MW2*
10 ml
50 ml
Buffer AVE
10 ml
20 ml


Storage and Stability

Proteinase K, DNase I powder and MagPure Particles N9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

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