Introduction
RNA Elution Buffer storage solution is a buffer with better RNA stability compared to TE buffer. The final step of each RNA isolation protocol is to resuspend the purified RNA precipitate. After extraction, it is crucial to suspend and store the particles in a safe, RNase free solution. RNA storage solutions have two characteristics that can minimize alkaline hydrolysis of RNA: low pH and sodium citrate (an efficient chelating agent). The RNA storage solution is compatible with all common RNA applications, such as reverse transcription, in vitro transcription, Northern analysis, and nuclease protection assays.
Formula
Formula: 1mm sodium citrate, pH 6.5
Prepare 1mm sodium citrate with ultrapure water, adjust to pH 6.5, add 0.1% DEPC and stir overnight, then package and sterilize.